An Experimental Study of Traditional Chinese Medicine Kangfuxin Inducing Apoptosis In Vitro of Peptic Carcinoma Cell Line BGC-823 / 昆明医科大学学报

ABSTRACT

Objective Investigate the apoptosis-inducing activity of Chinese medicine Kangfuxin(KFX)on human peptic carcinoma cell line BGC-823 and its related mechanism.Methods Cell proliferation in different concentration and time was determined by MTT(Methy thiazolyl tetrazolium) assay.The fluorescence flow cytometry(FCM) DNA assay and TUNEL(Terminal deoxynuxleotidel transferase mediated uridine nucleotide end labeling) were applied to detecting the changes of apoptotic rate at the early and the late apoptosis process,cell proliferation and alteration of cell cycle phase.Results After incubation of BGC-823 cells with different concentration of Kangfuxin for 24,48 and 72 hours,the IC50 were(17.85?1.06),(13.76?0.57)and(11.32?0.14)mg/mL(P