Effect of sodium nitroprusside on apoptosis in human airway smooth muscle cells of passive sensitization by serum from allergic asthmatic patients / 中国病理生理杂志

ABSTRACT

AIM:To investigate NO-donor sodium nitroprusside(SNP)-induced apoptosis of human airway smooth muscle cells(HASMCs) of passive sensitization by serum from allergic asthmatic patients.METHODS: The technique of human airway smooth muscle(HASMCs) passively sensitized with serum from allergic asthmatic patients was adopted.The effect of SNP on the survival rate of passively sensitized HASMCs was detected by MTT method.Apoptosis of cells was detected by TUNEL and flow cytometry.RESULTS:(1) Compared to sensitized group,the survival rate of passively sensitized HASMCs decreased in SNP+sensitized group(n=5,P