Effect of recombinant adenovirus vector of decorin on the expression of extracellular matrix molecules in rat mesangial cells / 中国病理生理杂志

ABSTRACT

AIM:To investigate the effect of adenovirus-medicated decorin gene transfer into rat mesangial cells(RMCs)on the expression of decorin,TGF-?1 and the extracellular matrix(ECM)components.METHODS:Rat mesangial cells were transfected with recombinant adenovirus vector of decorin,the levels of mRNA for decorin,TGF-?1,collagen type IV and fibronectin were estimated by semi-quantitative RT-PCR.RESULTS:The transfection of AD-DCN significantly increased decorin mRNA expression compared with negative control(P