Determination of Fluconazole in Human Plasma by RP-HPLC / 中国药房
China Pharmacy
;
(12)2007.
Artículo
en Chino
| WPRIM
| ID: wpr-529432
ABSTRACT
OBJECTIVE:
To establish RP-HPLC method for the determination of fluconazole in human plasma.METHODS:
After liquid-liquid extraction,the plasma sample was analyzed on Diamonsil C18 column with column temperature set at room temperature.The mobile phrase consisted of methanol-0.07% aqueous solution of triethylamine(50∶ 50) with a flow rate of 1mL? min-1.The UV detection wavelength was set at 210nm,and phenacetin was used as the internal standard.RESULTS:
The linear range of fluconazole was 0.15~ 10.0mg? L-1(r=0.999 2),with lower quantification limit at 0.15mg? L-1.The intra-day RSD was between 3.37% and 7.48% and the inter-day RSD was between 7.03% and 12.05%;the method recovery was 103.64%(RSD=5.04%),and the average extraction recovery was between 83.81%(RSD=5.77%).CONCLUSION:
This method was sensitive,accurate,rapid and reproducible,and suitable for the content determination of fluconazole in plasma.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
China Pharmacy
Año:
2007
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS