Changes in Mitochondrial Membrane Potential of K562 Cells in the Process of Apoptosis Induced by Simvastatin / 中国药房

ABSTRACT

OBJECTIVE:To observe the changes of mitochondrial membrane potential(MMP)at different time in the process of simvastatin-induced apoptosis of K562 cells.METHODS:K562 cells cultivated by routine method for 24h were treated in the 20? mol? L-1 simvastatin for 12,24,48,and 72h,respectively.And theh cell morphological changes were observed.Vitality of K562 cells was detected with MTT;flow cytometry were performed to detect cell apoptotic ratio and changes of mitochondrial membrane potential,and the detection results were compared with those in solvent control group.RESULTS:As compared with control group,the morphology changes of K562 cells such as karyopyconosis,nuclear fragmentation and apoptotic body emerged after treatment with 20? mol? L-1 simvastatin for 48h;the apoptotic ratio at 12,24,48,and 72h increased by(2.55? 0.35)%,(6.1? 0.35)%,(14.15? 0.42)%,and(30.70? 0.65)%,respectively;and the apoptotic ratio of K562 cells increased time-dependently.The cell membrane potential was decreased by(0.7? 0.24)%,(39.6? 4.80)%,(24.4? 2.45)%,and(6.0? 1.62)%,respectively,with that at 24h showing the highest decreasing rate.CONCLUSION:The change of MMP is the early events of apoptosis.The potential mechanism of K562 cells apoptosis induced by simvastatin might be related to the collapse of MMP.