Determination of Urine Level of Levofloxacin by Second-order Derivative Spectrometry / 中国药房

ABSTRACT

OBJECTIVE: To determine the urine level of levofloxacin by second-order derivative spectrophotometry.METHODS: The concentration of levofloxacin in human urine was determined at a wavelength of 350～ 360nm using a second-order derivative spectrum peak valley amplitude(P).RESULTS: The standard curve was linear in the concentration range of 2～ 18? g? mL-1(r=0.999 6).The average recovery was 100.2%(RSD=0.66%).The intra-day and inter-day RSD were 0.09% and 1.82%,respectively.CONCLUSION: The method is simple,stable,precise and reproducible,and in which blank urine sampling,the extraction and isolation were not needed,thus it can be used for the monitoring of urine drug level.