Simultaneous Determination of Notoginsenoside R_1 and Ginsenoside Rg_1 in Danqi Tablets by RP-HPLC / 中国药房

ABSTRACT

OBJECTIVE:To develop a RP-HPLC method for the simultaneous determination of Notoginsenoside R1 and Ginsenoside Rg1 in Danqi tablets. METHODS:The chromatographic separation was performed on a Kromasil C18(250mm?4.6mm,5?m) column with column temperature at room temperature. The mobile phase consisted of acetonitrile-water-H3PO4(20.5∶79.5∶0.02) at a flow rate of 1.0mL?min-1.The detection wavelength was set at 203nm. RESULTS:The linear range of Notoginsenoside R1 and Ginsenoside Rg1 were 4.67～46.68?g?mL-1 (r=0.999 0) and 4.62～115.50?g?mL-1 (r=0.999 9), respectively; with the average recover of Notoginsenoside R1 at 97.93% and that of Ginsenoside Rg1 at 97.98%, and RSD at 1.31%(n=6) and 1.38%(n=6), respectively. CONCLUSION:The method is simple, rapid and accurate, and suitable for the quality control of Danqi tablets.