Inhibitory effect of liposomes survivin antisense oligonucleotide on human hepatic carcinoma transplanted subcutaneously in nude mice / 中国病理生理杂志

ABSTRACT

AIM:To explore the effects of liposomes survivin antisense oligonucleotides(ASODN)on growth of human hepatic carcinoma transplanted subcutaneously in nude mice.METHODS:Nude mouse model of human hepatic cancer was established by transplantation of hepatic cancer cell line SMMC-7721/ADM subcutaneously.Models were divided randomly into six groups:control group,liposome group,sense oligonucleotide(SODN)group,200 ?g/L,400 ?g/L and 600 ?g/L ASODN groups.Different treatments were given respectively.Weight and volume of subcutaneous tumors were measured,and tumor growth inhibitory rate was calculated.Morphological changes of transplanted tumor cells were observed under light microscope.The expression of Survivin was detected by immunohistochemistry.RESULTS:The growth of tumors was significantly inhibits in all ASODN groups compared with control,liposome and SODN groups(P