A study on cytotoxicity of ADM-PBCA-NP to L-02 cells / 中国普通外科杂志

ABSTRACT

Objective To investigate the cytotoxicity of ADM-PBCA-NP on L-02 cells.Methods L-02 cells were cultured in vitro and the LDH activity of supernatant liquid of culture cells was examined.The toxicity of ADM-PBCA-NP,ADM and PBCA-NP to L-02 cells by the MTT assay was also determined,and the haemolysis function of PBCA-NP with different concentrations was detected.Results The cytotoxicity of ADM-PBCA-NP,ADM and blank PBCA nanoparticles to L-02 cells under the 10-6 mol/L concentration range was not cytototic(grade 1).LDH activity of supernatant liquid of culture cells showed no differences between the 3 groups.Conclusions Nanoparticles of ADM-NP and PBCA-NP in the 10-6 mol/L concentration range have no significant toxic effect on L-02 hepatic cells;and in a certain concentration range,the cell compatibility is excellent and will not lead to hemolysis.