Determination of the Content of Artemisia Annua L. Samples Planted in Different Site Condition in Chongqing Wulong County by UV-Vis / 中国药房

ABSTRACT

OBJECTIVE:To evaluate the quality of Artemisia annua L. samples planted in different site condition in Chongqing Wulong county and to provide a fast and convenient assaying of artemisinin in Artemisia annua L. samples in the industrial development of this Chinese herbal medicine. METHODS: The Artemisia annua L. planted in different site condition in Chongqing Wulong county were sampled during harvesting time, which were subjected to many processing steps such as drying by baking, crushing, microwave extraction and color reaction etc. Then the absorbance of the test liquid was detected by UV-spectrophotometry at a wavelength of 290 nm. Finally Artemisinin content was computed based on the regression curve of standard solution and the quality of the Artemisia annua L. samples was evaluated. RESULTS: The liner concentration range of Artemisinin was 8～40 mg?mL-1 (r=0.999 8) with an average recovery rate of 99.98% (RSD=1.48%, n=9).CONCLUSION: The results showed that site condition did have effect on the quality of Artemisia annua L. to some degree. UV-Vis method is simple, rapid and reproducible, and suitable for the quality control of Artemisia annua L. samples.