Determination of Osthol and Berberine in Kangfuyan Effervescent Suppository by RP-HPLC / 中国药房
China Pharmacy
;
(12)2005.
Artículo
en Chino
| WPRIM
| ID: wpr-530564
ABSTRACT
OBJECTIVE:
To establishes an HPLC method for determination of the contents of Osthol and Berberine in Kangfuyan effervescent suppository.METHODS:
HPLC was carried out on a column of Diamonsil (250 mm?4.6 mm,5 ?m) with mobile phase consisted of acetonitrile-0.2% phosphate acid (52∶48,Lauryl sulfonic acid sodium 0.1 g was contained in every 100 mL) with flow rate at 1 mL?min-1.The detection wavelength was set at 322 nm and the column temperature was set at room temperature.RESULTS:
The linear range was 0.019 8~0.396 0 ?g(r=0.999 97) for Osthole versus 0.056 65~1.133 00 ?g(r=0.999 99) for Berberine. The average recoveries of Osthol and Berberine were 101.06% (RSD=1.10%) and 101.88%(RSD=0.98%), respectively.CONCLUSION:
This method is simple, accurate and reproducible, and applicable for the quality control of Kangfuyan effervescent suppository.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
China Pharmacy
Año:
2005
Tipo del documento:
Artículo
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