Determination of Sildenafil Citrate Illegally Added in Kidney-yang Reinforcing Chinese Medicinal Tonics by RP-HPLC / 中国药房

ABSTRACT

OBJECTIVE: To determine the content of Sildenafil citrate illegally added in kidney-yang reinforcing Chinese medicinal tonics. METHODS: The samples were separated on ODS C18(250 mm?4.6 mm,5 ?m) with mobile phase consisted of methanol-water-acetic acid glacial-triethylamine(60∶40∶0.8∶0.3) at a flow rate of 0.8 mL?min-1.The UV detective wavelength was 290 nm and the sample size was 10 ?L. RESULTS: At a concentration range of 10～100 ?g?mL-1, Sildenafil citrate showed a good linear relation with peak area score. The mean recovery rate was 100.52%(RSD=1.20%). CONCLU-SIONThe method can be used to determine Sildenafil Citrate illegally added in kidney-yang tonics.