Determination of Oleanolic Acid in Ziyin shugan Granule by RP-HPLC / 中国药房
China Pharmacy
; (12)2005.
Article
en Zh
| WPRIM
| ID: wpr-531050
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WPRO
ABSTRACT
OBJECTIVE:To establish a RP-HPLC method to determine the content of Oleanolic acid in Ziyin shug-an granule. METHODS: Kromasil C18 column(250 mm?4.6 mm,5 ?m)was used as chromatographic column,and the mobile phrase consisted of methanol-0.2% phosphoric acid(80∶20) at a flow rate of 1.0 mL?min-1 and the det-ection wavelength was set at 208 nm,and the column temperature was maintained at room temperature.RESULTS:The calibration curve of Oleanolic acid was in good linearity over the range of 0.237~4.740 ?g(r=0.999 8).The average recovery of Oleanolic acid was 98.84% with RSD at 1.19%(n=6).CONCLUSION:The method is simple,feasible and reproducible, and can be used for the quality control of Ziyin shugan granule.
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Zh
Revista:
China Pharmacy
Año:
2005
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Article