Interferon and Verapamil for the Reversal of Multidrug Resistance in Breast Cancer Cell Line / 中国药房

ABSTRACT

OBJECTIVE: To investigate the effect of interferon-? and verapamil on the reversal of multidrug-resistance(MDR) in breast cancer cell line.METHODS: Drug-sensitive MCF-7 and ADM-induced MDR phenotypic MCF-7/ADR were taken as in vitro experimental models,which were treated with interferon-? and verapamil in combination or alone,then the cell survival rate,IC50,drug-resistant times and reversal times of different experiment groups were measured by MTT test.The quantitive expression of P-170 on the surface of cells was detected by flow cytometry.RESULTS: Treated by interferon-? and verapamil in combination,the IC50 of adriamycin-resistance was lowered to 0.32 ?mol?L-1,superior to verapamil(1.23 ?mol?L-1) or interferon-?(2.29 ?mol?L-1) alone;the reversal times increased to 51.88 in combination group,superior to interferon-? and verapamil alone(7.25 and 13.49 respectively) and the expression of P-170 were lower than in groups treated with interferon-? and verapamil alone.CONCLUSION: The efficacy of interferon-? and verapamil exhibited a more remarkable efficacy on the reversal of multidrug-resistance(MDR) in breast cancer cell line when used in combination than alone.