Metabolic loading of guanosine induces chondrocyte apoptosis via the Fas pathway
Experimental & Molecular Medicine
;
: 401-407, 2006.
Artículo
en Inglés
| WPRIM
| ID: wpr-53150
ABSTRACT
Although the apoptosis of chondrocytes plays an important role in endochondral ossification, its mechanism has not been elucidated. In this study, we show that guanosine induces chondrocyte apoptosis based on the results of acridine orange/ ethidium bromide staining, caspase-3 activation, and sub-G1 fraction analysis. The potent inhibitory effect of dipyridamole, a nucleoside transporter blocker, indicates that extracellular guanosine must enter the chondrocytes to induce apoptosis. We found that guanosine promotes Fas-Fas ligand interaction which, in turn, leads to chondrocyte apoptosis. These findings indicate a novel mechanism for endochondral ossification via metabolic regulation.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Glicoproteínas de Membrana
/
Transducción de Señal
/
Ratas Sprague-Dawley
/
Apoptosis
/
Receptores del Factor de Necrosis Tumoral
/
Receptor fas
/
Condrocitos
/
Proteínas de Transporte de Nucleósidos
/
Factores de Necrosis Tumoral
/
Proteína Ligando Fas
Límite:
Animales
Idioma:
Inglés
Revista:
Experimental & Molecular Medicine
Año:
2006
Tipo del documento:
Artículo
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