Effects of Soluble Cluster of Differentiation 14 on Serum Levels of Pro-and Anti-inflammatory Cytokines in Mice with Serious Scald Endotoxemia / 中国药房

ABSTRACT

OBJECTIVE:To study the effects of soluble cluster differentiation 14 (sCD14) on serum pro-and anti-inflammatory cytokines in mice with serious scald endotoxemia. METHODS: A total of 60 healthy Kunming mice were randomly assigned to sCD14 (5 mg?kg-1) group, treatment control group (normal saline) and normal control group (normal saline). The first two groups were established into scald model (scald of 30% body surface area, Ⅲ degree scald) followed by intravenous injection of the corresponding drugs in every 12 h via vena caudalis for 3 consecutive days. On day 4, the model mice were sacrificed to obtain the serum sample for the detection of levels of proinflammatory cytokines (TNF?, IL-6) and anti-inflammatory cytokines (IL-4 and IL-10). RESULTS: In sCD14 treatment group, serum levels of TNF?, IL-6 were significantly lower than in treatment control group (P