Effect of neuregulins on mtp53 and HIF-1? in MDA-MB-231 cells / 中国病理生理杂志

ABSTRACT

AIM:To explore the effect and significance of neuregulins /ErbB2 receptor signal transduction pathway on mtp53 and hypoxia-iducible factor-1?(HIF-1?) in none-overexpression ErbB2 breast cancer cell MDA-MB-231.METHODS:The expression of neuregulin was detected by immunocytochemistry and Western blotting.MDA-MB-231 cells were treated with ErbB2 kinase inhibitor AG825.Proliferation was measured by MTT assay.The cell cycle and apoptosis were determined by flow cytometry.The expressions of mtp53 and HIF-1? were detected by Western blotting.The mRNA expression of HIF-1? was detected by RT-PCR.RESULTS:MDA-MB-231 cells expressed a relative higher level of neuregulin.In the results of Western blotting,the positive reaction band was found in 44 kD which coincides with the molecular weight of neuregulin.When MDA-MB-231 cells were treated with AG825,the proliferation was inhibited in time and dose dependent manners(P