Apoptosis Expression-based Pattern Recognition System of Four Properties of Chinese Materia Medica——Panax quinque folium vs. Panax ginseng in Drug Property against MPP~+-induced Apoptosis of SH-SY5Y Cells / 中国药房

ABSTRACT

OBJECTIVE:To investigate the effects of water extract of Panax quinque folium(WEAG,of cool property)and Panax ginseng(WERG,of warm property)on MPP+-induced apoptosis of SH-SY5Y cells.METHODS:SH-SY5Y cells were divided into 4 groupsthe control group was cultured in complete medium,model group was cultured with 0.5 mmol MPP+;Panax quinque folium group was treated with 0.5 mmol MPP+ plus different concentration of WEAG,and Panax ginseng group was treated with 0.5 mmol MPP+ and different concentration of WERG concomitantly.WEAG and WERG were added for intervention.the morphology of SH-SY5Y cells was observed by Hoechst33258 staining and the apoptotic rate was detected by FCM;the cell survival rate was measured by MTT chromatometry.RESULTS:Compared with model group,WEAG and WERG markedly reversed the apoptotic characteristics of SH-SY5Y cells,markedly up-regulated MTT value and lowered the apoptotic rate,with WERG showing more potent efficacy than WEAG doing.CONCLUSION:Water extracts of both Panax quinque folium(cool)and Panax ginseng(warm)pose protective effects of different degree against MPP+-induced apoptosis of SH-SY5Y cells,with the Chinese materia medica of warm property showing more potent action against apoptosis.Chinese materia medicas of different drug property have different anti-apoptotic action.