Antihyperglycemic Activity of Herba Taraxaci Polysaccharides / 中国药房

ABSTRACT

OBJECTIVE:To study the antihyperglycemic activity of Herba Taraxaci polysaccharides.METHODS:1,1-diphenyl-2-picryl-2-picrylhydrazyl(DPPH) radical scavenging and FRAP assays were used to analyze the antioxidant activity of Herba Taraxaci polysaccharides;the inhibitory activity of ?-glucosidase in vitro was tested by micromethod; the diabetic mice model was established using alloxan. After intragastric administration of Herba Taraxaci polysaccharide for 14 days,the fasting serum glucose and antioxidant parameters (SOD,MDA and GSH-Px) in dissected liver tissue were assayed. RESULTS:The TEAC values assayed by DPPH and FRAP methods were 564.98 ?molTE?g-1 and 1 399.55 ?molTE?g-1,respectively; and the IC50 value of Herba Taraxaci polysaccharides against ?-glucosidase was 49.27 ?g?mL-1.Herba Taraxaci polysaccharides had no effect on serum polysaccharides and antioxidant parameters in normal mice,but which significantly down-regulated the fasting serum glucose of diabetic mice especially at a high dosage(P