Content Determination of Main Component in Intravenous Emulsion of Vinorelbine by HPLC / 中国药房

ABSTRACT

OBJECTIVE:To establish an HPLC method for determination of the content of main component in intravenous emulsion of vinorelbine. METHODS: The content of vinorelbine was determined by HPLC on Gemini C18 column with mobile phase consisted of acetonitrile-0.06 mol?L-1 KDP buffer (pH was adjusted to 3.0 by hydrochloric acid)(35∶65) at a flow rate of 0.8 mL?min-1. The detection wavelength was set at 215 nm;the column temperature was set at 30 ℃,and the injection volume was 5 ?L. RESULTS: The linear range of vinorelbine was 0.08～0.80 mg?mL-1 (r=0.999 9) and the average recovery rate was 99.33% with intra-day RSD at 0.95% and inter-day RSD at 1.14%. CONCLUSION: The method is simple,accurate and reproducible,and it is applicable for the determination of the intravenous emulsion of vinorelbine.