Effect of Vinblastine on the Proliferation and Nitric Oxide Secretion of RAW264.7 Cells / 中国药房

ABSTRACT

OBJECTIVE:To explore the effect of vinblastine(VBL)on the proliferation and nitric oxide(NO)secretion of RAW264.7 cells. METHODS:MTT and flow cytometry were used to evaluate the inhibition effect of VBL on the proliferation of RAW264.7 cells and blockade effect of VBL on cell cycle distribution,respectively. The change of protein expression of cyclin B1 was detected by Western blot method. Griess kit was adopted to test the effect of VBL on LPS- induced NO secretion of cell. RESULTS:VBL can inhibit the proliferation and NO secretion of RAW264.7 cells,arrest cells in G2～M phase and reduce the levels of cyclin B1 in a concentration- dependent manner(P