Poly(I:C) promotes iodine excess-induced chronic lymphocytic thyroiditis in NOD mouse / 中国病理生理杂志

ABSTRACT

AIM:To investigate the effects of poly (IC) as virus mimics on iodine excess-induced chronic lymphocytic thyroiditis in NOD mouse. METHODS:Female,32 NOD mice were randomly divided into 4 groups(1) control; (2) high iodine; (3) poly (IC); (4) high iodine+poly (IC). Nine weeks after administration,mice were sacrificed. The following parameters were determinedbody weight,thyroid weight and anatomic form. Thyroid hormone (T4) in serum was measured by radioimmunoassay,the thyroid morphology was observed through HE staining,apoptosis was detected by TUNEL,the mRNA expression levels of TRAIL,TRAIL-sR1,ICAM-1 and CXCL10 were determined by the method of real time RT-PCR. RESULTS:Compared to control group and poly (IC) group,the thyroid absolute weight and relative weight in high iodine group were increased (P0.05). CONCLUSION:Poly (IC) aggravates chronic lymphocytic thyroiditis induced by excess of iodine associated with increase in infiltration of lymphocytes and induction of apoptosis.