Simultaneous Determination of Imperatorin and Isoimperatorin in Yuanhu Zhitong Tablet by Capillary Electrophoresis / 中国药房
China Pharmacy
;
(12)2005.
Artículo
en Chino
| WPRIM
| ID: wpr-533894
ABSTRACT
OBJECTIVE:
To establish capillary electrophoresis method for the determination of imperatorin and isoimperatorin in Yuanhu zhitong tablet.METHODS:
The separation was performed on a uncoated fused silica capillary (55 cm?75 ?m ID,effective length of 47 cm) with 20 mmol?L-1 NaH2PO4+100 mmol?L-1 SDS+80%formamide as buffer solution (pH=7.12). The separation voltage was 15 kV and the sample was injected by gravity (5 s,15 cm). The detection wavelength was 254 nm.RESULTS:
The linear range of imperatorin and isoimperatorin were 8.0~40.0 ?g?mL-1(r=0.998 7) and 4.0~20.0 ?g?mL-1(r=0.999 0). The average recovery was 101.3% for imperatorin (RSD=3.24%,n=6) and 100.2% for isoimperatorin(RSD=1.44%,n=6).CONCLUSION:
The method is convenient,rapid and accurate for the quality control of Yuanhu zhitong tablet.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
China Pharmacy
Año:
2005
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS