Effect of Aldosterone on the Proliferation and Collagen Metabolism of Cardiac Fibroblasts in Neonate Rat / 中国药房

ABSTRACT

OBJECTIVE: To investigate the effects of aldosterone (Ald) on the proliferation and collagen synthesis of cardiac fibroblasts (CFs) in neonate rats and to research its mechanism. METHODS: The neonatal rat cardiac fibroblasts were induced by anchorage velocity-dependent separation method. SD rats were divided into control group, Ald (1.0?10-7 mol?L-1) group, Ald combined with spirolactone group and Ald combined with losartan group. After 24 h of exposure, CFs proliferation was measured by MTT assay and cell cycle distribution was determined with flow cytometer (FCM). Spectrophotography was used to detect the content of hydroxyproline and immunofluorescence staining method was applied to determine the protein expression of proliferation cell nuclear antigen (PCNA) and fibronectin (FN). RESULTS: As compared with control group, in Ald group the activity of CFs was increased as well as its percentage in S stage. The content of hydroxyproline and the protein expression of PCNA and FN were also increased while the percentage of CFs in G0/G1 stage was decreased (P0.05). CONCLUSION: Ald-stimulated CFs proliferation and collagen synthesis were mediated by activation of AT1 receptors and Ald receptor,and Ald receptor plays a major role. A corresponding increase in the protein expression of PCNA and FN in CFs may be related to the proliferation effect.