Simultaneous Determination of Peoniflorin, Campanulin and Ferulic Acid in Kangmin Granules by HPLC / 中国药房
China Pharmacy
;
(12)2005.
Artículo
en Chino
| WPRIM
| ID: wpr-534187
ABSTRACT
OBJECTIVE:
To establish an HPLC method for simultaneous determination of peoniflorin,calycosin and ferulic acid in Kangmin granules.METHODS:
The separation was performed on a Kromasil C18 column (250 mm?4.6 mm,5 ?m),and the mobile phase consisted of methanol-acetonitrile-0.4% phosphonic acid (20 ∶ 10 ∶ 70) with flow rate of 1.0 mL?min-1.The detection was performed at wavelength of 254 nm.Column temperature was room temperature and the injection volume was 10 ?L.RESULTS:
The linear ranges of peoniflorin,calycosin and ferulic acid were 180~3 600 ?g?mL-1(r=0.999 2),4.6~92.0 ?g?mL-1(r=0.999 1) and 8.0~160.0 ?g?mL-1(r=0.999 4),respectively.The average recoveries were 98.3%(RSD=1.8%,n=9),99.0% (RSD=2.0%,n=9) and 100.5%(RSD=1.1%,n=9),respectively.CONCLUSION:
The method is simple,accurate,sensitive and reproducible for the quality control of Kangmin granules.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
China Pharmacy
Año:
2005
Tipo del documento:
Artículo
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