Protective Effect of Serum Containing Shangjining Capsules on Myocardial Cell Damage Induced by Adriamycin / 中国药房

ABSTRACT

OBJECTIVE: To study protective effect of serum containing Shangjining (SJN) capsules on myocardial cell damage induced by adriamycin (ADR).METHODS:Primary culture of myocardial cell in neonatal SD rat was conducted and ADR-inducing myocardial cell damage model were established.Biochemical index of supernatant fluid were detected and flow cytometry was used to determine the apoptosis of myocardial cell.RESULTS: ADR increased the content of supernatant fluid LDH and MDA while decreased the activity of SOD.High,middle and low dose of SJN capsule can decreased the activity of LDH and the content of MDA and increased the activity of SOD.Results of flow cytometry showed SJN could reduce cell apoptosis.CONCLUSION: SJN capsule could inhibit ADR-inducing peroxidation of myocardial cell free radical and cell apoptosis and protect myocardial cell damage.