Research on Anti-penicilloyl Group Monoclonal Antibody Establishment of Hybridoma Cell Lines Secreting High Titer of Anti-Penicilloyl Group Monoclonal Antibodies / 中国免疫学杂志

ABSTRACT

14 hybridoma cell lines were established by fusing spleen cells of BALB/c mouse immunized with BPO_(21)-BSA with SP2/o myeloma cells. Fusing rates are 100% and antibody secreing positive rate are 14.5% and 16.7% and followed by cloning the positive rate incresing to 100% were obtained. 14 hybridoma cell lines prepared ascites that were positive at 8?10~7-2.6?10~7 respectively as measured by ELISA method, the specific of antibodies were proved by ELISA and inhibition ELISA method with BPO23-HSA. The chromosome number of these 6 hybridomas cell are 98 to 115.One out of 14 hybridomas cell line is IgG2b, the others are belong to IgG1. These kinds of anti-penicilloyl group antibodies will be used to detecting the allergen in penicillin preparation.