Inhibitory effect of maie inhibitory material fraction 1 on B lymphocytes / 中国免疫学杂志

ABSTRACT

Human seminal plasma contains male inhibitory material (MIM) and ther suppressive factors which prevent the induction of immune reaction to sperm and seminal plasma. In this paper, the MIM was purified from li(?)uified healthy human ejaculates by Sephadex G-100 filtration. As previously shown, five peaks were isolated and only one band was demonstrated on SDS-PAGE in fraction 1 (MIMF-1). MIMF-(?) was found to have inhibitory effect on immune response to sRBC in mice as detected by haemolytic plaque method in vitro, and also be able to reduce the antibody titers to sRBC in murine serum. The possible relationship between immune suppression caused by MIMF- 1 and hypersensitivity to seminal fluid or sterity was strongly suggested.