Kinetic analysis on production of IL-1, IL-6 and TNF? by human monocytes / 中国免疫学杂志

ABSTRACT

Kinetics of production of IL-1, IL-6 and TNFa induced by endotoxin from human monocytes as well as their relationships were studied using cytokine bioassays and immu nocytochemical staining methods. The results showed that accu mulation of IL-1 ?, IL-6 and TNFa molecules in cytoplasma and their release into supernatants had different kinetic patterns from each others, reflecting the difference in gene activation, transcription, translation and release of these three cytokines. In addition, only part of monocytes synthesize IL-1 ?, IL-6 and TNF?, suggesting the presence of different cytokineproducing monocyte subsets.