Expression of recombinant human interleukin 8 in E.coll and its functional identification / 中国免疫学杂志

ABSTRACT

By using genetic engineering techniques, we had successed in the expression of recombinant human interleukin 8 (rhIL-8)in E. col(?) IL-8 cDNA insert into the plasmid pGEM-IL-8 was subcloned into expression vector pMS316, and IL-8 fusion protein was produced in E.coli with high efficiency, The purified recombinant protein exhibited chemotactic activity for mouse neutrophils both in vive and in vitro. It showed that a fragment of the human IL-8 had no influence on the chemotactic activity of IL-8.