Effects of SRBC immunization on beta-adrenoceptors in lymphocytes isolated from the spleens of rats / 中国免疫学杂志

ABSTRACT

The Bmax and Kd of beta-adrenoceptors in splenie lymphocytes of rats were determined by direct binding of radioligand, ~3H-dihydroalpranolol. Bmax of beta-adrenoceptors was significantly increased on day+1, was peak on day+2 and gradually reduced to control values on day+ 6 after the intraperitoneal injection of 5?10~9SRBC. A positive correlation (r=0. 86) was found between the Bmax of beta-adrtenoceptors on day+1 to+3 and the amount of Ig M in s(?)rum after 72 hours. Both the Bmax of beta-adrenoceptors on day+2 and the amount of Ig M in serum on day+5 were reduced by the intraperitoneal injection of propranolol. In study on antibody response to SRBC.in vitro, Ig M Synthesis in mouse spleen cells was enhanced by norepinephrine (10?mol/L), and blocked by propranolol (1?mol/L). These results indicated that (1) the immune response to SRBC in vivo up-regulates the density of beta-adrenoceptors in splenic lymphocytes; (2) the up-regulation of Bmax of beta-adrenceptors in splenic lymphocytes may increase its Ig M synthesis;(3) the enhancement of the Ig M antibody response to SRBC in vitro by norepinephrine was mediated by the beta-adrenoceptors.