Cloning and sequencing of mouse immunoglobulin kappa variable domains against human small lung cancer by the polymerase chain reaction / 中国免疫学杂志

ABSTRACT

We have directly amplified immunoglobulin kappa variable domains from the genomic DNA of 2F7 ybridoma against human small cell lung cancer by the polymerase chain reaction(PCR)with a set of universal primers that were on the FR1 site and FR4 site of V_(?) genesrespectively.By EcoRI and BglⅡ,the DNA fragments amplified were force-cloned into pGEM-7Zf(+)-V_kPCR that carried human immunoglobulin kappa constant domain.By sequencing,It conformed that we had got the V_(?) gene that was 318 bp in length.