THE METHODS DETECTING VASCULAR ENDOTHELIAL GROWTH FACTOR IN HUMAN BREAST CARCINOMAS / 中国现代普通外科进展

ABSTRACT

Objective: To establish analysis method of vascular endothelial growth Factor(VEGF)for clinic practice.Methods: the expression of VEGF in breast carcinoma tissue is examined with immunohistochemistry, RT-PCR, Western blot and dot blot. Results: the VEGF is revealed mainly in tumor cells and extracellular matrix, molecular weight of VEGF is 46 KD, including three isoforms. In 53% of the cases the tumor tissue reveals stronger expression of VEGF than paratumor tissue. Conclusion:Dot blot may be used as a preliminary method for detecting VEGF in breast carcinoma tissue.