Establishment of detecting methods of Th1/Th2 cytokine gene / 中国免疫学杂志
Chinese Journal of Immunology
;
(12)1999.
Artículo
en Chino
| WPRIM
| ID: wpr-535565
ABSTRACT
Objective:
TO establish methods for sensitive, rapid, economical detection of Th1/Th2 Cytokine gene expression.Method:
sixpairs of Primers specific for the IL-2, IL-4, IL-10, IL-13, IFNr and B-actin were designed. A Sensitive and rapid method for detection of Th1/Th2cytokine genes by RT-RCR was established. Meanwhile anothermethod:
the RNA hybridization with sir cDNA probes were established.Results:
The RT-PCR reaction and RNA hybridization was used to test five kinds of Cytokine mRNAs expressed 20 tumor cell lines on mRNAs were expressed in eleven Cell lines;The mRNAs in eight cell lines.Conclusion:
The preliminary results suggested that RT-PCR and RNA hybridizationnight be used to test Th1/Th2 cytokine gene expression.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Immunology
Año:
1999
Tipo del documento:
Artículo
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