Preparation of sgp130 ELISA kit and its application / 中国免疫学杂志
Chinese Journal of Immunology
;
(12)1985.
Artículo
en Chino
| WPRIM
| ID: wpr-536673
ABSTRACT
Objective:
To establish the sensitive,specific,stable and convenient sgp130 ELISA kit.Methods:
The mAb T2 against human gp130 was used as coating antibody;the other mAb T12 recognized different epitope with T2 was labeled by biotin,then a ELISA kit for detecting sgp130 was set up.Results:
sgp130 ELISA kit is successfully established and its sensitivity is 10 ng/ml.After the kit is placed in 4℃ for 3 months,the kit's CV is less than ?7.6% and the retrievable rate is 95%~111%.These indicate that it has highly sensitivity,stability and accuracy.The normal serum concentration of sgp130 in healthy donors is 536.92~287.88(ng/ml),but there is higher in patients with hyperthyroidism(937.16?217.5) and chronic nephritis(806.45?138.47).Significant difference is found comparing with normal control(P
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Immunology
Año:
1985
Tipo del documento:
Artículo
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