Expression of activating transcription factor 5(ATF5) in renal cell carcinoma / 中华泌尿外科杂志

ABSTRACT

ObjectiveTo investigate the differential expression of ATF5--a novel gene fished out by yeast two hybrid from renal carcinoma library.MethodsRT-PCR and Northern blot was used to examine the expression level of ATF5 in renal cell line A498,786-O,GRC-I,293 and HK-2.The area absorbance value of ATF5 RNA was quantitative by computerized-imaging system.ResultsCompared with the controls,the expression level in renal cancer cell line(A498,786-O,GRC-I) and embryonal cell line (293)is much higher than that in the normal cell line HK-2.ConclusionsIt is indicated that ATF5 may play a significant role in the renal carcinogenesis through cross-talk with Wnt signal pathway.