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The study of biological activity of the expression products of the human ?-NGF cDNA in CHO cell line and purified the seperated protein / 中国免疫学杂志
Chinese Journal of Immunology ; (12)1985.
Artículo en Chino | WPRIM | ID: wpr-537981
ABSTRACT

Objective:

To detect the biological activity of the expression products of the human ?-NGF cDNA in CHO cell line and purified the seperated protein.

Methods:

Human ?-NGF was transfected with lipofectamine reagent into CHO cell line.The biological activity was analyzed by objection with microscope and the method of MTT.The pure protein was proved by SDS-PAGE analysis.

Results:

The protein in the culture supernatant of the positive CHO cells transfected with ?-NGF gene could stimulate the growth of PC12 cell line and go into BBB.

Conclusion:

The target gene expressed successfully in the transfected CHO cell line and had good biological activity. [

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: Chinese Journal of Immunology Año: 1985 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: Chinese Journal of Immunology Año: 1985 Tipo del documento: Artículo