Dynamic changes of rat donor mycardial cell apoptosis during hypothermic preserv ation and the protective effect of melatonin / 中华器官移植杂志

ABSTRACT

ObjectiveTo study the dynamic changes of rat donor myocardial cell apoptosis during hypot hermic preservation and the effect of melatonin.MethodsEighty rats were randomly divided into two groups experimental group with the i solated rat hearts being preserved in 4?℃ St Thomas solution with melatonin ( 0.1?mmol/L ); control group with the isolated rat hearts being preserved in 4?℃ St Thomas solution only. After preservation for 4, 8, 12, 24 and 36?h, a poptotic index was evaluated by the percentage of mycardial cells using TUNEL po sitive staining.ResultsWith the hypothermic preservation time prolonging, more myocardial cell apoptosi s was found (control group, P