PAGE-AgNO_3 staining displays AP-PCR fingerprint of mutans streptococci / 实用口腔医学杂志
Journal of Practical Stomatology
;
(6)1996.
Artículo
en Chino
| WPRIM
| ID: wpr-539349
ABSTRACT
Objective:
To investigate whether the AP-PCR fingerprint of mutans streptococci(MS) can be displayed by PAGE-AgNO 3 staining.Methods:
Amplification products of 200 MS clinical isolates by AP-PCR was separated and stained by ?=3.5%PAGE-AgNO 3 and agarose-EB respectively. Results were compared and the agreement value of Kappa between two methods was calculated.Results:
?=3.5%PAGE-AgNO 3 discerned both homogeneity and heterogeneity of MS genotypes, just as agarose-EB,Kappa value for agreement was 1.00 . Moreover, more bands was showed by PAGE-AgNO 3 staining than by agarose-EB, so PAGE-AgNO 3 gave a clearer pattern than agarose-EB.Conclusion:
AP-PCR fingerprint of MS can be displayed by ?=3.5%PAGE-AgNO 3 staining.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Journal of Practical Stomatology
Año:
1996
Tipo del documento:
Artículo
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