Differentially expressed genes of hypertrophic scar and normal skin / 中华医学美学美容杂志

ABSTRACT

Objective To screen the differentially expressed genes between hypertrophic scar of burn and normal skin using gene microarray, to find out the genes of significantly different-expression and to analyse the roles of them in development of hypertrophic scar. Methods The total DNA and RNA from 4 hypertrophic scar samples and 4 normal skin were isolated and purified to mRNA by oligotex. They were reversely transcribed to cDNA with the incorporation of fluorescent dTUP to prepare the hybridization probes. Then, the mixed probes were hybridized to the cDNA chip and scanned for the signals and found differences between scar and normal skin. Results Among 4000 target genes, there were 378～451 genes once and 114～152 third times significantly different between hypertrophic scar and normal skin and 97 different-expressed genes in all 4 cases. Conclusions There are differences of gene expression between hypertrophic scar and normal skin screened by microarray. As the scanned cases increase, the common differentially-expressed genes become less and less, which may involve in the development of hypertrophic scar.