A three-dimensional model of angiogenesis in vitro / 中国现代普通外科进展
Chinese Journal of Current Advances in General Surgery
;
(4)1999.
Artículo
en Chino
| WPRIM
| ID: wpr-539792
ABSTRACT
Objective:
To develop an experimental three-dimensional model by ECV304 cells(human umbilical vein endothelial cell line) for investigating the mechanisms of angiogenesis in vitro.Methods:
ECV304 cells were seeded onto three-dimensional collagen gels made of rat-tail collagen.When the endothelial cells were cultured and grown to near confluence,treated with bFGF for 3 to 12 days,and then assessed with inverted phase contrast microscope.Results:
The endothelial cells migrated into the gels,formed complex networks by cell cords at different levels through the bottom view,and sprouted capillary-like structures through the side view.Conclusion:
ECV304 cells are capable of expressing some early events of angiogenesis in the three-dimensional collagen gelsproliferating,migrating and sprouting and so on.It should be useful for studying angiogenesis in vitro.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Current Advances in General Surgery
Año:
1999
Tipo del documento:
Artículo
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