Differentiation induction of dental mesenchymal cells into odontoblasts / 实用口腔医学杂志

ABSTRACT

Objective: To induce human dental mesenchymal cells to differentiate into odontoblasts in vitro.Methods:The cultured human dental mesenchymal cells were induced in two-dimensional culture model by bFGF(10 ng/ml)+IGF-1(100 ng/ml) or TGF-?1(5 ng/ml) for 4-7 d. Cell growth and morphology after induction were observed. The expression of human DSP protein and DSPP mRNA were detected by immunofluorescent staining and RT-PCR. Mineralization capability of the induced cells was evaluated using Von Kossa staining. Results:In both bFGF+IGF and TGF-?1 groups 20%-30% of the induced cells showed long single process.DSP protein and DSPP mRNA were observed in the induced cells.Mineralized nodules were found in the induction cultures.Conclusion: bFGF+IGF-1 or TGF-?1 can induce dental mesenchymal cells to differentiate into odontoblasts.