Treatment of human PC-3 pancreatic cancer with Kanglaite combined with 5-Fu——an experimental study on subcutaneous transplantation tumor nude mouse / 西安交通大学学报(医学版)

ABSTRACT

Objective To investigate the mechanism of Kanglaite combined with 5-Fu in treating pancreatic carcinoma through studying the changes of Bcl-2,Bax and vascular endothelial growth factor(VEGF) in pancreatic carcinoma cell and the relationship between apoptosis and these changes.Methods The model of athymic mouse subcutaneous transplantation tumor of human pancreatic carcinoma PC-3 cell was established;the expression of Bcl-2,Bax and VEGF were detected in malignant cells using immunohistochemistry;the apoptosis proportion was detected by adopting flow cytometry;the tumor size was detected.Results Kanglaite combined with 5-Fu could obviously depress the expression of Bcl-2 in protein level in malignant cells;apoptosis proportion had no effect on Bax expression;Kanglaite could obviously depress the expression of VEGF in malignant cells.The apoptosis proportion was higher and protein expression of Bcl-2 was obviously lower in group of Kanglaite combined with 5-Fu than that in Kanglaite group or 5-Fu group.What was more,the protein expression of VEGF and the tumor volum was lowest in group of Kanglaite combined with 5-Fu.Conclusion The effect of Kanglaite united with 5-Fu on treatment of pancreatic cancer is better than that of Kanglaite or 5-Fu alone.