Expression and significance of neuronal nitric oxide synthase in ureteropelvic junction obstruction / 中华泌尿外科杂志

ABSTRACT

Objective To study the expression and significance of neuronal nitric oxide synthase(nNOS) in ureteropelvic junction obstruction(UPJO).Methods Specimens of the narrow segment of the ureteropelvic junction(UPJ) were obtained from 36 cases of UPJO(without aberrant vessels,adhesive band compression and high insertion of ureteral junction).Furthermore,the light microscopic examination demonstrated that smooth muscle fascicles were sparse and thin,and fibrous connective tissues between them were deposited.Control UPJ specimens were taken from 20 cases of renal neoplasm,and tissues were confirmed histologically to be unaffected.Immunohistochemical staining(SABC method) was performed to detect the expression of nNOS in specimens of the 2 groups.Quantitative analysis was made using image analysis technique. Results Immunoreactivity to nNOS was predominantly present in the smooth muscle cell cytoplasm of UPJ.The mean density of nNOS in UPJO and control groups was 0.208?0.014 and 0.230?(0.016),respectively,which were significantly different between the 2 groups(P