Effects of Warming and Tonifyin Kidney-Yang Drugs on Liver Mitochondria Proteome of Rats with Kidney-Yang Deficiency / 中华中医药杂志

ABSTRACT

Objective: To study the effects of warming and tonifying kidney-Yang drugs on the liver mitochondria proteome of rats with kidney-yang deficiency using two-dimensional difference gel electrophoresis(2-D DIGE).Methods: Every liver mitochondria protein sample from the normal rats,the kidney-yang deficiency rats and the kidney-yang deficiency rats treated with warm and tonify kidney-yang drugs were labeled with Cy3 or Cy5 randomly,Each Cy3-labeled sample and Cy5labeled sample were mixed on the same 2-D gel along with a Cy2-labeled mixture of all samples as an internal standard and run on the same gel.All images were analyzed by DeCyder software DIGE technology with internal standard.16 unique proteins were selected,and in which 10 protein were successfully identified.Results: In the kidney-yang deficiency rats,the expressions of carbamoyl-phosphate synthetase 1,dihydrolipoamide branched chain transacylase,ubiquinol-cytochrome c reductase,isovaleryl coenzyme A dehydrogenase,heat shock protein 60,succinyl-CoA synthetase,hydroxyamino-coenzyme A dehydrogenase/ ketoacyl coenzyme acetyl coenzyme A dehydrogenase ?subunit,ornithine transcarbamylase and glutathione peroxidase all increased.While the expression of ornithine aminotransferase decreased.After the treatment of warming and tonifying kidney-yang drugs,the expressions of above-mentioned proteins all had rectification in different degrees. Conclusion: The metabolism of carbohydrate,lipids and protein had been rectified when the kidney-yang deficiency rats were fed with warming and tonifying kidney-yang drugs,which was likely attributed to the improvement of stability of mitochondria membrane.