Biological responses of osteoblast-like cells in different maturation stages under shear flow / 中国矫形外科杂志
Orthopedic Journal of China
; (24)2006.
Article
en Zh
| WPRIM
| ID: wpr-543594
Biblioteca responsable:
WPRO
ABSTRACT
[Objective]To sturdy the biological responses of osteoblast-like cells in different maturation stages under shear flow.[Method]Flow chamber system was used to apply shear flow stimulation of 4.62dyn?s/cm~2 for 2 hours on osteoblast-like cell MC3T3-E1 of different maturation stage induced by osteogenic media,and then samples for dsDNA content and ALP activity analysis were collected.[Result]On 24 hours after simulation,there was not obvious change of ALP activity with non-osteogenic inducing group,an increase was observed in the group of 4 days of differentiation,while an decrease in the group of 8 days of differentiation.[Conclusion]Biological responses of osteoblast-like cells in different maturation stages under shear flow is quite different,and this may be an important role in bone growth and reconstruction.
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WPRIM
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Zh
Revista:
Orthopedic Journal of China
Año:
2006
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Article