Study of effect of recombinant human IFN-?-2b bacilli Calmette-Gu?rin in PBMCs expressing cytokines / 中华泌尿外科杂志

ABSTRACT

Objective Measure the cytokines including hIFN-?、hIL-12 and hTNF-? which secreted by PBMC stimulated with recombinant BCG, and research how recombinant BCG influence the expression of cytokines and improve the immunologic response. Methods In the experiment, Recombinant hIFN-?-2b-BCG group and wild-type BCG group stimulate peripheral blood monocytes(PBMC) with different density in vitro, which is 0.1 OD and 0.01 OD (1 OD=2.7?107CFU). In third group, we put IFN-?-2b into wild-type BCG so that we can compare it with recombinant BCG. All these components foster with PBMC(4?106/ml), then collect the supernatant in 12 hours, 24 hours, 48hours, 72 hours, 5 day, 7day, and detect hIFN-?、hTNF-?, hIL-12 by an enzyme-linked immynosorbent assay(ELISA), compare the results. Results We can learn from the result, compared with the other groups, recombinant BCG can induce higher density cytokines than wild-type BCG’s and combination group’s(P