Distribution of Polymorphisms and Linkage of GSTM1 and GSTM3 Genes in Healthy Cantonese in Southern China / 环境与健康杂志

ABSTRACT

Objective To determine the prevalence of the polymorphisms of GSTM1 and GSTM3 genes in target population in Guangdong province and to investigate the linkage between the two genes and their association with the family history distribution of the target population. Methods Polymerase chain reaction-restriction (PCR)-2% agarose gel electrophoresis and PCR-12% PAGE electrophoresis approaches were employed to detect the genotype of the GSTM1 and GSTM3. The data was analyzed with SPSS10.0 software. Results The frequency of GSTM1(-) genotype was 56.8%(n=597). The frequency of genotype of GSTM3*A-*A,*A-*B,*B-*B were 62.3%,25.8%,11.9% respectively. Conclusion A linkage between the GSTM1 and GSTM3 gene is showed in the present paper. The analysis of logistic regression shows that the expression of homozygote for GSTM3*B-*B gene is correlated with the family history of coronary heart disease in the target population.