Combined Effects of p,p'-DDE and ?-BHC on Lipid Peroxidation in Rat Sertoli Cells in vitro / 环境与健康杂志

ABSTRACT

Objective To study the combined effects of p,p'-DDE and ?-BHC on lipid peroxidation in the rat Sertoli cells in vitro. Methods After separated Sertoli cells from testicular tissue of rats and treated with the toxicants at different doses, p,p'-DDE(10, 30, 50 ?mol/L), ?-BHC(10, 30, 50 ?mol/L) and p,p'-DDE+?-BHC(10+10, 30+30, 50+50 ?mol/L) which were decided by MTT method, the leakage of LDH, the activity of SOD and the contents of MDA in Sertoli cells were determined. Results The absorbance values of 50 ?mol/L p,p'-DDE and ?-BHC were significantly decreased (P