Content determination of Total Saponins from Rubus parvifolius L / 重庆医科大学学报

ABSTRACT

Objective; To establish a method for content determination of total saponins in Rubus parvifolius L.. Methods; Total saponins was determined by UV - VIS spectrophotometry after different extracts of the sample had been coloured. Results; The method was linear in the range of 22-176?g (r = 0.9997),and the average recovery was 102. 1 % , RSDwas 2. 82% (n = 6). The content of total saponins was highest by the method of 70% ethanol reflux. Conclusion; The sample is stable after coloured. The method is sensitive and reliable , which may be used for the quality control of Rubus parvifolius L. .